EFFECT OF MILLIMETER RANGE ELECTROMAGNETIC WAVES ON COMPLEX-FORMATION OF ETHIDIUM BROMIDE AND HOECHST 33258 WITH DNA
نویسندگان
چکیده
منابع مشابه
Interaction of Hoechst 33258 and ethidium with histone1-DNA condensates.
We report structural and dynamical aspects of DNAs from various sources including synthetic oligonucleotides in bulk buffer and as a complex with histone1 (H1). High-resolution transmission electron microscopic (HRTEM) studies reveal the structural change of the DNAs upon complexation with H1 leading to formation of compact-globular and hollow-toroidal particles. In order to explore the functio...
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An essential element of cellular and molecular biology is the ability to quantitate DNA in large numbers of samples at a sensitivity that enables determination of small amounts of sample. Here we describe a fluorescent method to quantitate DNA with Hoechst 33258 dye and the BioTek FL600 fluorescence microplate reader. This assay provides linear results over three orders of magnitude and can rou...
متن کاملBinding Mechanisms of Methylene Blue and Hoechst 33258 with Dna
In this work the data of methylene blue (MB) and Hoechst 33258 interaction with DNA are presented. It has been shown that at 0.002 M ionic strength of solution MB is bound to double-stranded DNA by three modes: intercalation, semi-intercalation and electrostatic. It was revealed that at higher ionic strengths of solution complete intercalation of MB becomes thermodynamically unacceptable. It wa...
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چکیده ندارد.
15 صفحه اولDifferent Modes of Binding of Hoechst 33258 with Dna
Spectrophotometric study of Hoechst 33258 binding to DNA has been investigated. It was shown that this ligand forms at least two types of complexes at 0.002 M and 0.02M ionic strengths of solution. One of them is strong, the other is weak. It was revealed that at 0.02M ionic strength of solution, the binding constant value by strong mode is one order higher than at 0.002M. It was obtained that ...
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ژورنال
عنوان ژورنال: Proceedings of the YSU B: Chemical and Biological Sciences
سال: 2020
ISSN: 1829-1767
DOI: 10.46991/pysu:b/2020.54.2.125